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doi: 10.2337/db05-0585

Biotinylated proteins eluted from the z13 beads were detected by SDSpolyacrylamide gel electrophoresis (PAGE) followed by peroxidase avidin blot and an ECL (Amersham, Piscataway, NJ) chemiluminescence reaction

It is important to check for coexistent folate and iron deficiency
