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Induction of apoptosis was detected by Propidium Iodide (PI, 1 g/mL) (Sigma Aldrich) and Annexin-V-APC (Immunotools) staining, followed by flow cytometry analysis, using a BD FACSCantoII flow cytometer
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Over time, an untreated leaky mitral valve puts strain on the heart and lungs, potentially causing complications such as an enlarged left atrium, atrial fibrillation (irregular heartbeat), or high blood pressure in the lung vessels ( pulmonary hypertension )
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